Detailed view for LmjF.30.0810

Basic information

TDR Targets ID: 26606
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2409 | Length (AA): 250 | MW (Da): 28157 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05891   AdoMet dependent proline di-methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0006480   N-terminal protein amino acid methylation  
GO:0008168   methyltransferase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 250 1xtp (A) 4 254 99.00 0 1 2.26 -2.05
4 250 1xtp (A) 2 254 98.00 0 1 2.2396 -1.81

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1XTP:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127629)

Species Accession Gene Product
Arabidopsis thaliana AT5G44450   alpha N-terminal protein methyltransferase 1
Brugia malayi Bm1_20180   Hypothetical 26.1 kDa protein in POP4-SHM1 intergenic region
Candida albicans CaO19_7069   hypothetical protein
Candida albicans CaO19.7069   similar to S. cerevisiae YBR261C
Caenorhabditis elegans CELE_Y74C9A.3   Protein HOMT-1
Cryptosporidium hominis Chro.60155   hypothetical protein
Cryptosporidium parvum cgd6_1210   hypothetical protein
Dictyostelium discoideum DDB_G0269658   hypothetical protein
Drosophila melanogaster Dmel_CG1675   N-terminal methyltransferase
Echinococcus granulosus EgrG_000411200   alpha N terminal protein methyltransferase 1A
Entamoeba histolytica EHI_036850   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000411100   alpha N terminal protein methyltransferase 1A
Echinococcus multilocularis EmuJ_000410700   nuclear pore complex protein nup214
Echinococcus multilocularis EmuJ_000411200   alpha N terminal protein methyltransferase 1A
Giardia lamblia GL50803_12215   S-adenosylmethionine-dependent methyltransferase, putative
Homo sapiens ENSG00000148335   N-terminal Xaa-Pro-Lys N-methyltransferase 1
Homo sapiens ENSG00000203740   methyltransferase like 11B
Leishmania braziliensis LbrM.30.0940   hypothetical protein, conserved
Leishmania donovani LdBPK_300880.1   AdoMet dependent proline di-methyltransferase, putative
Leishmania infantum LinJ.30.0880   hypothetical protein, conserved
Leishmania major LmjF.30.0810   hypothetical protein, conserved
Leishmania mexicana LmxM.29.0810   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05539   hypothetical protein
Mus musculus 240879   methyltransferase like 11B
Mus musculus ENSMUSG00000026857   N-terminal Xaa-Pro-Lys N-methyltransferase 1
Neospora caninum NCLIV_023020   Methyltransferase like 11A, related
Oryza sativa 4334312   Os03g0780900
Saccharomyces cerevisiae YBR261C   Tae1p
Schistosoma japonicum Sjp_0000060   similar to UPF0351 protein C9orf32, putative
Schistosoma mansoni Smp_124220   hypothetical protein
Trypanosoma brucei gambiense Tbg972.6.2090  
Trypanosoma brucei gambiense Tbg972.6.2050   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.2330   RGG protein
Trypanosoma brucei Tb927.6.2270   AdoMet dependent proline di-methyltransferase, putative
Trypanosoma congolense TcIL3000_0_37880   RGG protein
Trypanosoma cruzi TcCLB.503835.30   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.509049.20   hypothetical protein, conserved
Toxoplasma gondii TGME49_201720   protein c9orf32, putative
Trichomonas vaginalis TVAG_452370   protein C9orf32, putative
Trichomonas vaginalis TVAG_127860   conserved hypothetical protein
Trichomonas vaginalis TVAG_095110   conserved hypothetical protein
Trichomonas vaginalis TVAG_361900   conserved hypothetical protein
Trichomonas vaginalis TVAG_253130   conserved hypothetical protein

Essentiality

LmjF.30.0810 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2270 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.2270 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.2270 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.2270 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y74C9A.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y74C9A.3 Caenorhabditis elegans larval lethal wormbase
CELE_Y74C9A.3 Caenorhabditis elegans sterile wormbase
TGME49_201720 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Lmaj004091AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Lmaj004091; Recombinant protein: full-length; Source: L major; SAM-dependent methyltransferase of the DUF858/Pfam05891 family ;

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LmjF.30.0810 (Leishmania major), hypothetical protein, conserved
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